1FMV

CRYSTAL STRUCTURE OF THE APO MOTOR DOMAIN OF DICTYOSTELLIUM MYOSIN II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-06-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	103.500, 179.700, 54.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.100
R-factor	0.196
Rwork	0.196
R-free	0.28000
RMSD bond length	0.013 *
RMSD bond angle	2.680 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.100
Rmerge	0.053
Total number of observations	202575 *
Number of reflections	59189
<I/σ(I)>	20
Completeness [%]	99.0	98 *
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.5	277	8% Peg 8000, 50 mM Hepes, 1 mM DTT, pH 7.5, microbatch, temperature 4K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	8.0 (%(w/v))
2	1	1	HEPES	50 (mM)
3	1	1	dithiothreitol	1 (mM)
4	1	1	protein	5.0 (mg/ml)