1FM5
CRYSTAL STRUCTURE OF HUMAN CD69
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2000-03-18 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 48.200, 48.200, 117.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.270 |
| Rwork | 0.243 |
| R-free | 0.29900 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.062 | 0.306 |
| Total number of observations | 36807 * | |
| Number of reflections | 13674 * | |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 96.3 | 86.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | drop was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 5 | 1 | reservoir | zinc acetate | 0.2 (M) | |
| 6 | 1 | reservoir | PEG1000 | 20 (%) |
