1FM0

MOLYBDOPTERIN SYNTHASE (MOAD/MOAE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	95
Detector technology	CCD
Collection date	1999-01-01
Detector	ADSC QUANTUM 4
Spacegroup name	C 1 2 1
Unit cell lengths	66.029, 48.955, 76.457
Unit cell angles	90.00, 107.72, 90.00

Refinement procedure

Resolution	10.000 - 1.450
R-factor	0.1532
Rwork	0.152
R-free	0.18200
RMSD bond length	0.013
RMSD bond angle	1.382
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.510
High resolution limit [Å]	1.450	1.450
Rmerge	0.054 *	0.454
Total number of observations	426992 *
Number of reflections	43689
<I/σ(I)>	27.9
Completeness [%]	95.6 *	89.5
Redundancy	4.5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	295	Sodium chloride, Hepes , pH 7.5, VAPOR DIFFUSION, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		5 (M)
2	1	reservoir	HEPES	0.1 (M)