1FL1
KSHV PROTEASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-08-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 53.580, 53.580, 323.060 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.000 - 2.200 |
| R-factor | 0.2253 |
| Rwork | 0.225 |
| R-free | 0.25970 |
| RMSD bond length | 0.561 |
| RMSD bond angle | 3.800 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.282 | 2.220 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.062 | 0.351 |
| Number of reflections | 28034 | |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 298 | 22% PEG-2K, 100 mM Tris-HCl, 10% glycerol, 190 mM LiSO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 25K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1.2 (mg/ml) | |
| 10 | 1 | drop | 76 (mM) | ||
| 11 | 1 | reservoir | PEG2000 | 22 (%) | |
| 12 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 13 | 1 | reservoir | glycerol | 10 (%) | |
| 14 | 1 | reservoir | 190 (mM) | ||
| 2 | 1 | drop | t-Boc-YLKA-chloromethyl ketone | 0.2 (mM) | |
| 3 | 1 | drop | acetate | 2.0 (%) | |
| 4 | 1 | drop | potassium phosphate | 10 (mM) | |
| 5 | 1 | drop | glycerol | 4 (%) | |
| 6 | 1 | drop | dithiothreitol | 0.4 (mM) | |
| 7 | 1 | drop | PEG2000 | 8.8 (%) | |
| 8 | 1 | drop | Tris-HCl | 40 (mM) | |
| 9 | 1 | drop | glycerol | 4 (%) |
