1FKQ
RECOMBINANT GOAT ALPHA-LACTALBUMIN T29V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 282.5 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-08 |
| Detector | MACSCIENCE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.750, 59.380, 45.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.189 * |
| Rwork | 0.189 |
| R-free | 0.24100 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.256 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.092 | 0.375 |
| Number of reflections | 10970 | |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 90.4 | 87.8 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 20 * | PEG 4000, ammonium sulfate, calcium chloride, potassium phosphate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30-40 (mg/ml) | |
| 2 | 1 | drop | 1 (mM) | ||
| 3 | 1 | reservoir | PEG8000 | 16-20 (%) | |
| 4 | 1 | reservoir | potassium phosphate | 0.05 (M) | pH6.0 |
| 5 | 1 | reservoir | 1 (mM) |
