1FK6
STRUCTURAL BASIS OF NON-SPECIFIC LIPID BINDING IN MAIZE LIPID-TRANSFER PROTEIN COMPLEXES WITH ALPHA-LINOLENIC ACID REVEALED BY HIGH-RESOLUTION X-RAY CRYSTALLOGRAPHY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-11-28 |
| Detector | ENRAF-NONIUS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.040, 50.360, 69.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.27400 |
| Structure solution method | MR |
| Starting model (for MR) | (PDB CODE:1MZL) |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.700 |
| Data reduction software | PROFILE-FITTING (PROCEDURE) |
| Data scaling software | PROFILE-FITTING (PROCEDURE) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.047 |
| Total number of observations | 18547 * |
| Number of reflections | 6996 |
| Completeness [%] | 92.6 |
| Redundancy | 2.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 24.5-25.5 * | 4.4M Na formate, 0.1M bezene sulfornic acid, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium formate | 4.4 (M) | |
| 2 | 1 | reservoir | benzene sulfonic acid | 0.1 (M) |
