1FK0

STRUCTURAL BASIS OF NON-SPECIFIC LIPID BINDING IN MAIZE LIPID-TRANSFER PROTEIN COMPLEXES WITH CAPRIC ACID REVEALED BY HIGH-RESOLUTION X-RAY CRYSTALLOGRAPHY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1999-01-01
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	24.750, 49.870, 69.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.214 *
Rwork	0.214
R-free	0.24100
Structure solution method	MR
Starting model (for MR)	(PDB code:1mzl)
RMSD bond length	0.017
RMSD bond angle	1.700
Data reduction software	PROFILE-FITTING (PROCEDURE)
Data scaling software	PROFILE-FITTING (PROCEDURE)
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall
High resolution limit [Å]	1.760
Rmerge	0.029
Total number of observations	23393 *
Number of reflections	8379
Completeness [%]	89.2
Redundancy	2.79

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4 *	24.5-25.5 *	3.3M Na formate, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	100 (mM)
2	1	reservoir	trisodium citrate	1.2 (M)
3	1	reservoir	sodium formate	3.3 (M)
4	1	reservoir	glycerol	10 (%(v/v))