1FJJ
CRYSTAL STRUCTURE OF E.COLI YBHB PROTEIN, A NEW MEMBER OF THE MAMMALIAN PEBP FAMILY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-06-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 85.470, 85.470, 73.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.660 |
| R-factor | 0.18 * |
| Rwork | 0.180 |
| R-free | 0.19681 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.026 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.750 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.045 * | 0.440 * |
| Total number of observations | 165834 * | |
| Number of reflections | 35266 * | |
| <I/σ(I)> | 13.6 | 1.7 |
| Completeness [%] | 96.2 | 76.3 |
| Redundancy | 4.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 298 | Sodium citrate, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | dithiothreitol | 2 (mM) | |
| 4 | 1 | reservoir | trisodium citrate dihydrate | 1.25-1.40 (M) | |
| 5 | 1 | reservoir | HEPES | 0.1 (M) |
