Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-05-01 |
| Detector | BRANDEIS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 100.079, 129.462, 143.738 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 2.680 |
| Rwork | 0.226 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gaf PDB ENTRY 1OSP |
| RMSD bond length | 0.009 |
| RMSD bond angle | 26.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.780 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.048 | 0.112 |
| Total number of observations | 221681 * | |
| Number of reflections | 51741 * | 5136 * |
| <I/σ(I)> | 19.9 | |
| Completeness [%] | 97.8 | 98.4 |
| Redundancy | 4.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.15 | 277 | 100 mM sodium cacodylate, 100 mM sodium acetate, 10 % (w/v) PEG 3350, pH 6.15, VAPOR DIFFUSION, HANGING DROP, temperature 4K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 4 | 1 | reservoir | PEG3350 | 10 (%(w/v)) |
