1FIV
STRUCTURE OF AN INHIBITOR COMPLEX OF PROTEINASE FROM FELINE IMMUNODEFICIENCY VIRUS
Experimental procedure
| Collection date | 1994-09-02 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 50.650, 50.650, 74.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.148 |
| Rwork | 0.148 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.047 * |
| Data reduction software | XENGEN |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.060 |
| Total number of observations | 17555 * |
| Number of reflections | 7033 |
| Completeness [%] | 90.0 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3-5 (mg/ml) | |
| 2 | 1 | drop | imidazole-HCl | 50 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | MPD | 5-10 (%) | |
| 6 | 1 | reservoir | MPD | 20-25 (%) | |
| 7 | 1 | reservoir | imidazole-HCl | 50 (mM) |
