1FIA
CRYSTAL STRUCTURE OF THE FACTOR FOR INVERSION STIMULATION FIS AT 2.0 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.390, 50.980, 79.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.000 |
| R-factor | 0.192 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 22.500 * |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Total number of observations | 44050 * |
| Number of reflections | 8221 * |
| Completeness [%] | 92.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.2 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | FIS | 20-30 (mg/ml) | |
| 2 | 1 | drop | 0.5 (M) | ||
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | Tris-HCl | 10 (mM) | |
| 5 | 1 | reservoir | sodium/potassium phosphate | 1.5 (M) |
