1FI2

CRYSTAL STRUCTURE OF GERMIN (OXALATE OXIDASE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	1999-07-11
Detector	ADSC QUANTUM 4
Spacegroup name	H 3 2
Unit cell lengths	95.593, 95.593, 107.109
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.400 - 1.600
Rwork	0.196
R-free	0.23300
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	0.024
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.070	0.083
Number of reflections	23707
<I/σ(I)>	24.4
Completeness [%]	95.4	85.3
Redundancy	14	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3 *	18 *	Woo, E.J., (1998) FEBS Lett., 437, 87. *
1	VAPOR DIFFUSION, HANGING DROP	5.3 *	18 *	Woo, E.J., (1998) FEBS Lett., 437, 87. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (mg/ml)
2	1	reservoir	ammonium sulfate	2 (M)
3	1	reservoir	2-propanol	5 (%)
4	1	reservoir	glycerol	20 (%)	cryoprotectant
5	1	reservoir	2-propanol	8 (%)	cryoprotectant