1FI2
CRYSTAL STRUCTURE OF GERMIN (OXALATE OXIDASE)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-07-11 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 95.593, 95.593, 107.109 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.400 - 1.600 |
| Rwork | 0.196 |
| R-free | 0.23300 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.024 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.070 | 0.083 |
| Number of reflections | 23707 | |
| <I/σ(I)> | 24.4 | |
| Completeness [%] | 95.4 | 85.3 |
| Redundancy | 14 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 * | 18 * | Woo, E.J., (1998) FEBS Lett., 437, 87. * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 * | 18 * | Woo, E.J., (1998) FEBS Lett., 437, 87. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 3 | 1 | reservoir | 2-propanol | 5 (%) | |
| 4 | 1 | reservoir | glycerol | 20 (%) | cryoprotectant |
| 5 | 1 | reservoir | 2-propanol | 8 (%) | cryoprotectant |
