1FHV
CRYSTAL STRUCTURE ANALYSIS OF O-SUCCINYLBENZOATE SYNTHASE FROM E. COLI COMPLEXED WITH MG AND OSB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-07 |
| Detector | APS-1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 71.800, 83.000, 57.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.770 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.22400 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 16.030 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.045 | 0.112 |
| Total number of observations | 319215 * | |
| Number of reflections | 33959 * | |
| <I/σ(I)> | 16.3 | |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 5.4 * | 296 | 5% Peg 4000, 3% Ethylene glycol,15 mM MgCl2, 1 mM OSB, 25 mM MES, microbatch, temperature 23K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 20 (mg/ml) | |
| 2 | 1 | 1 | PEG4000 | 5 (%) | |
| 3 | 1 | 1 | ethylene glycol | 3 (%) | |
| 4 | 1 | 1 | 15 (mM) | ||
| 5 | 1 | 1 | OSB | 1 (mM) | |
| 6 | 1 | 1 | MES | 25 (mM) |
