1FHU
CRYSTAL STRUCTURE ANALYSIS OF O-SUCCINYLBENZOATE SYNTHASE FROM E. COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-12-07 |
| Detector | SBC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.100, 70.000, 80.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.650 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.26500 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 16.110 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.069 | 0.294 |
| Number of reflections | 36924 * | |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 99.0 | 98.4 |
| Redundancy | 11.4 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.75 | 296 | 27% ME-PEG 5000, 267 mM NaCl, 50 mM Mes, 15-20 mg/ml protein, pH 6.75, micro batch, temperature 23K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 15-20 (mg/ml) | |
| 2 | 1 | 1 | mPEG5000 | 27 (%) | |
| 3 | 1 | 1 | 267 (mM) | ||
| 4 | 1 | 1 | MES | 50 (mM) |
