1FHN
TRANSTHYRETIN STABILITY AS A KEY FACTOR IN AMYLOIDOGENESIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-11-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.770, 86.170, 65.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.750 |
| R-factor | 0.1534 |
| Rwork | 0.153 |
| R-free | 0.18050 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.063 | 0.450 |
| Total number of observations | 101000 * | |
| Number of reflections | 25047 * | |
| <I/σ(I)> | 20 | |
| Completeness [%] | 98.4 | 99.3 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.3 * | 287 | Damas, A.M., (1996) Acta Crystallog., D52, 966. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 41 (%) | |
| 3 | 1 | reservoir | sodium citrate | 200 (mM) |
