1FHL

CRYSTAL STRUCTURE OF BETA-1,4-GALACTANASE FROM ASPERGILLUS ACULEATUS AT 293K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-03-14
Detector	RIGAKU RAXIS II
Spacegroup name	I 2 2 2
Unit cell lengths	60.420, 88.940, 129.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.900 * - 2.300
R-factor	0.174
Rwork	0.166
R-free	0.24500
RMSD bond length	0.008
RMSD bond angle	1.400 *
Data reduction software	TRUNCATE
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	SHARP
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.900 *	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.096	0.392
Number of reflections	15582
<I/σ(I)>	7.1
Completeness [%]	97.5	95.6
Redundancy	4.6	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	Ryttersgaard, C., (1999) Acta Crystallogr.,Sect.D, 55, 929. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG400	30 (%(v/v))
2	1	reservoir		0.2 (M)
3	1	reservoir	sodium HEPES	0.1 (M)	pH7.5