1FHH

X-RAY CRYSTAL STRUCTURE OF OXIDIZED RUBREDOXIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	1999-06-27
Detector	FUJI
Spacegroup name	H 3
Unit cell lengths	64.115, 64.115, 32.183
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.500
R-factor	0.197
RMSD bond length	0.030 *
RMSD bond angle	3.570 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	10.000
High resolution limit [Å]	1.500	1.500
Rmerge	0.059	0.059
Number of reflections	8577
Completeness [%]		96

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.6	277	2M ammonium sulfate, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.0 (M)
2	1	reservoir	sodium acetate	0.1 (M)