1FGY
GRP1 PH DOMAIN WITH INS(1,3,4,5)P4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 41.300, 107.000, 36.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.500 |
| Rwork | 0.213 |
| R-free | 0.24700 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.058 | 0.327 |
| Number of reflections | 24163 | |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 94.0 | 85.3 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.8 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | complex | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 6-8 (%) | |
| 3 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 4 | 1 | reservoir | 100 (mM) | ||
| 5 | 1 | reservoir | glycerol | 10 (%) |
