1FGX

CRYSTAL STRUCTURE OF THE BOVINE BETA 1,4 GALACTOSYLTRANSFERASE (B4GALT1) CATALYTIC DOMAIN COMPLEXED WITH UMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	1998-09-17
Detector	CUSTOM-MADE
Spacegroup name	C 2 2 21
Unit cell lengths	108.500, 161.000, 107.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.280 - 2.400
R-factor	0.225 *
Rwork	0.225
R-free	0.26800
RMSD bond length	0.016
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	DM
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.280	2.550
High resolution limit [Å]	2.400	2.400
Rmerge	0.058	0.350
Total number of observations	91528 *
Number of reflections	34096
<I/σ(I)>	9.2
Completeness [%]	96.0	95 *
Redundancy	2.8	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5 *	20 *	PEG 5000, Tris, MnCl2, Li2SO4, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-15 (mg/ml)
2	1	drop	Tris Maleate	20 (mM)
3	1	drop		2 (mM)
4	1	drop		50 (mM)
5	1	reservoir	PEG5000	25-30 (%)
6	1	reservoir		100-150 (mM)
7	1	reservoir	Tris	100 (mM)