1FGV

X-RAY STRUCTURES OF FRAGMENTS FROM BINDING AND NONBINDING VERSIONS OF A HUMANIZED ANTI-CD18 ANTIBODY: STRUCTURAL INDICATIONS OF THE KEY ROLE OF VH RESIDUES 59 TO 65

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	64.200, 61.300, 51.800
Unit cell angles	90.00, 99.00, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.18
Rwork	0.180
RMSD bond length	0.013
RMSD bond angle	2.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	1.890 *
Rmerge	0.231 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ammonium acetate	0.2 (M)
3	1	reservoir	sodium acetate	0.1 (M)
4	1	reservoir	PEG3400	15 (%(w/v))