1FGM
LIPOXYGENASE-1 (SOYBEAN) AT 100K, N694H MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-03 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.260, 92.770, 49.230 |
| Unit cell angles | 90.00, 90.22, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.189 |
| Rwork | 0.178 |
| R-free | 0.21240 |
| Structure solution method | IR |
| Starting model (for MR) | 1f8n |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.471 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 | 0.312 |
| Number of reflections | 65373 | |
| <I/σ(I)> | 29.1 | |
| Completeness [%] | 96.6 | 84.5 |
| Redundancy | 3.07 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | PEG3350, Sodium Acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
