1FGL

Cyclophilin A complexed with a fragment of HIV-1 GAG protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	44.300, 53.100, 68.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.195
Rwork	0.195
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	2.800
Data reduction software	R-AXIS
Data scaling software	R-AXIS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.000
High resolution limit [Å]	1.800 *	1.800
Rmerge	0.068
Total number of observations	58111 *
Number of reflections	14651
Completeness [%]	94.2	88.3
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	50 (mM)
2	1	drop	ammonium sulfate	6 (%sat)
3	1	drop	CyPA	0.32 (mM)
4	1	reservoir	Tris-HCl	90 (mM)
5	1	reservoir	ammonium sulfate	41 (%sat)
6	1	reservoir	sodium azide	4.5 (mM)