1FGK
CRYSTAL STRUCTURE OF THE TYROSINE KINASE DOMAIN OF FIBROBLAST GROWTH FACTOR RECEPTOR 1
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02-01 |
| Detector | FUJI |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 208.350, 57.780, 65.570 |
| Unit cell angles | 90.00, 107.23, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.26100 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 22.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.44) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.048 | 0.197 |
| Total number of observations | 122569 * | |
| Number of reflections | 50771 | |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 97.3 | 96.3 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 4 * | 16% PEG 10000, 0.3 M (NH4)2SO4, 100 MM BIS-TRIS, PH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20-50 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | PEG10000 | 16 (%) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.3 (M) | |
| 7 | 1 | reservoir | ethylene glycol | 5 (%) | |
| 8 | 1 | reservoir | bis-Tris | 100 (mM) |
