1FGJ

X-RAY STRUCTURE OF HYDROXYLAMINE OXIDOREDUCTASE

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	297
Detector technology	IMAGE PLATE
Collection date	1996-05-06
Spacegroup name	P 63
Unit cell lengths	96.200, 96.200, 265.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.23
Rwork	0.230
R-free	0.30900
Structure solution method	MIRAS
RMSD bond length	0.012 *
RMSD bond angle	1.629 *
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	PHASES
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.970
High resolution limit [Å]	2.800	2.800
Rmerge	0.790	0.166
Number of reflections	33905
Completeness [%]	99.8	95
Redundancy	12.2	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8		pH 8.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	ammonium sulfate	1.2 (M)
3	1	drop	Tris-HCl	25 (mM)
4	1	reservoir	ammonium sulfate	2.4 (M)
5	1	reservoir	Tris-HCl	50 (mM)