1FGG
CRYSTAL STRUCTURE OF 1,3-GLUCURONYLTRANSFERASE I (GLCAT-I) COMPLEXED WITH GAL-GAL-XYL, UDP, AND MN2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-30 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.842, 48.219, 102.204 |
| Unit cell angles | 90.00, 92.79, 90.00 |
Refinement procedure
| Resolution | 24.780 - 2.300 |
| R-factor | 0.188 * |
| Rwork | 0.188 |
| R-free | 0.21500 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.064 | 0.243 |
| Total number of observations | 69398 * | |
| Number of reflections | 24398 * | |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 97.3 | 82.5 |
| Redundancy | 2.8 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 296 | monomethyl ether PEG2000, MnCl2, MgCl2, UDP-GlcA, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 23K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG2000 MME | 21 (%) | |
| 2 | 1 | reservoir | MES | 0.1 (M) | |
| 3 | 1 | drop | protein | 15 (mg/ml) | |
| 4 | 1 | drop | HEPES | 25 (mM) | |
| 5 | 1 | drop | 50 (mM) | ||
| 6 | 1 | drop | 5 (mM) | ||
| 7 | 1 | drop | 5 (mM) | ||
| 8 | 1 | drop | UDP-GlcUA | 10 (mM) |
