1FGA

REFINEMENT OF THE STRUCTURE OF HUMAN BASIC FIBROBLAST GROWTH FACTOR AT 1.6 ANGSTROMS RESOLUTION AND ANALYSIS OF PRESUMED HEPARIN BINDING SITES BY SELENATE SUBSTITUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	rotating-anode X-ray tube
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Detector	AREA DETECTOR
Spacegroup name	P 1
Unit cell lengths	30.800, 33.500, 35.800
Unit cell angles	58.80, 72.40, 76.10

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.138
RMSD bond length	0.018
RMSD bond angle	3.200
Data scaling software	Xengen (HOWARD, NIELSEN, XUONG)
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	2.200 *
High resolution limit [Å]	1.600 *
Rmerge	0.040 *
Number of reflections	13971 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.1 *		using macroseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	2	ammonium sulfate	2.0 (M)
2	1	2	Tris-HCl	0.1 (M)
3	1	2		0.1 (M)
4	1	2	BME	0.1 (%(v/v))