1FFW

CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY WITH A BOUND IMIDO DIPHOSPHATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1997-10-14
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	159.300, 53.400, 77.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.700
R-factor	0.213 *
Rwork	0.213
R-free	0.27300
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.065	0.191
Number of reflections	16044
<I/σ(I)>	13.5
Completeness [%]	85.9	88.8
Redundancy	2.2	2.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	297	Welch, M., (1998) Nature Struct. Biol., 5, 25. *