1FFN
CRYSTAL STRUCTURE OF MURINE CLASS I H-2DB COMPLEXED WITH PEPTIDE GP33(C9M)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2000-03-15 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 |
Unit cell lengths | 47.258, 68.913, 81.751 |
Unit cell angles | 72.92, 73.24, 69.97 |
Refinement procedure
Resolution | 50.000 - 2.700 |
Rwork | 0.240 |
R-free | 0.28600 |
RMSD bond length | 0.008 |
RMSD bond angle | 24.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.870 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.119 | 0.354 |
Total number of observations | 99827 * | |
Number of reflections | 22352 | |
<I/σ(I)> | 5.2 | |
Completeness [%] | 89.8 | 85.6 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 14% PEG8000, 1% Dioxane, 25mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | MES | 25 (mM) | pH6.5 |
3 | 1 | reservoir | PEG8000 | 10-20 (%) | |
4 | 1 | reservoir | MES | 25 (mM) | pH6.5 |
5 | 1 | reservoir | dioxane | 1 (%) |