1FFE
CONTRIBUTION OF CUTINASE SERINE 42 SIDE CHAIN TO THE STABILIZATION OF THE OXYANION TRANSITION STATE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1993-12-31 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.120, 67.360, 37.050 |
| Unit cell angles | 90.00, 93.90, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.690 |
| R-factor | 0.135 |
| Rwork | 0.135 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.690 * |
| Rmerge | 0.039 |
| Total number of observations | 56063 * |
| Number of reflections | 17069 |
| Completeness [%] | 89.9 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 100 (mM) | |
| 2 | 1 | reservoir | PEG6000 | 10-20 (%) |
