1FF9
APO SACCHAROPINE REDUCTASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-05-19 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 114.986, 56.584, 74.299 |
Unit cell angles | 90.00, 111.09, 90.00 |
Refinement procedure
Resolution | 24.910 - 2.000 |
R-factor | 0.241 * |
Rwork | 0.241 |
R-free | 0.27300 |
RMSD bond length | 0.006 |
RMSD bond angle | 22.600 * |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.910 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.053 | 0.330 |
Total number of observations | 578167 * | |
Number of reflections | 29843 * | |
<I/σ(I)> | 25.5 | |
Completeness [%] | 98.5 | 98.3 |
Redundancy | 19.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.8 | 4 * | ammonium sulphate, sodium acetate, DTT, pH 4.8, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 2.5 (M) | |
3 | 1 | reservoir | sodium acetate | 0.1 (M) | |
4 | 1 | reservoir | dithiothreitol | 1 (mM) |