1FF9

APO SACCHAROPINE REDUCTASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-05-19
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	114.986, 56.584, 74.299
Unit cell angles	90.00, 111.09, 90.00

Refinement procedure

Resolution	24.910 - 2.000
R-factor	0.241 *
Rwork	0.241
R-free	0.27300
RMSD bond length	0.006
RMSD bond angle	22.600 *
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.910	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.053	0.330
Total number of observations	578167 *
Number of reflections	29843 *
<I/σ(I)>	25.5
Completeness [%]	98.5	98.3
Redundancy	19.4	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.8	4 *	ammonium sulphate, sodium acetate, DTT, pH 4.8, VAPOR DIFFUSION, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	ammonium sulfate	2.5 (M)
3	1	reservoir	sodium acetate	0.1 (M)
4	1	reservoir	dithiothreitol	1 (mM)