1FF9
APO SACCHAROPINE REDUCTASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-19 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.986, 56.584, 74.299 |
| Unit cell angles | 90.00, 111.09, 90.00 |
Refinement procedure
| Resolution | 24.910 - 2.000 |
| R-factor | 0.241 * |
| Rwork | 0.241 |
| R-free | 0.27300 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.600 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.910 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.053 | 0.330 |
| Total number of observations | 578167 * | |
| Number of reflections | 29843 * | |
| <I/σ(I)> | 25.5 | |
| Completeness [%] | 98.5 | 98.3 |
| Redundancy | 19.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.8 | 4 * | ammonium sulphate, sodium acetate, DTT, pH 4.8, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 2.5 (M) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 4 | 1 | reservoir | dithiothreitol | 1 (mM) |
