1FE6

CRYSTAL STRUCTURE OF A NATURALLY OCCURING PARALLEL RIGHT-HANDED COILED-COIL TETRAMER

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	110.243, 110.243, 70.932
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.1977 *
Rwork	0.198
R-free	0.21650 *
RMSD bond length	0.004
RMSD bond angle	0.720 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		30.000
High resolution limit [Å]	1.800 *	1.800
Rmerge	0.052 *
Total number of observations	854694 *
Number of reflections	46116 *
Completeness [%]	100.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.9 *	4 *	ammonium sulphate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12.8 (mg/ml)
2	1	reservoir	ammonium sulfate	2 (M)
3	1	reservoir	Tris-HCl	200 (mM)	pH7.9