1FDR

FLAVODOXIN REDUCTASE FROM E. COLI

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1994-03-08
Detector	RIGAKU
Spacegroup name	C 1 2 1
Unit cell lengths	124.130, 51.100, 40.670
Unit cell angles	90.00, 93.10, 90.00

Refinement procedure

Resolution	8.000 - 1.700
R-factor	0.184
Rwork	0.184
R-free	0.24800
Structure solution method	MIR
RMSD bond length	0.018
RMSD bond angle	25.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.000
High resolution limit [Å]	1.700	1.700
Rmerge	0.064 *	0.230 *
Total number of observations	68969 *
Number of reflections	28503
<I/σ(I)>	17.8	3.3
Completeness [%]	96.0	29
Redundancy	2.5	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.3	20 *	1.2 M SODIUM CITRATE 100MM TRISHCL PH 7.3

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	sodium citrate	1.2 (M)
3	1	reservoir	Tris-HCl	100 (mM)