1FDO

OXIDIZED FORM OF FORMATE DEHYDROGENASE H FROM E. COLI

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	1995-10
Detector	RIGAKU RAXIS
Spacegroup name	P 41 21 2
Unit cell lengths	146.600, 146.600, 81.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.800
R-factor	0.192
Rwork	0.192
R-free	0.28100
Structure solution method	DIFFERENCE FOURIER ANALYSIS USING PHASES FROM THE STRUCTURE OF REDUCED FDH-H (PDB ENTRY 1AA6)
RMSD bond length	0.013
RMSD bond angle	24.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.079	0.200
Total number of observations	84166 *
Number of reflections	20861
<I/σ(I)>	22.1	8.4
Completeness [%]	93.4	91.4
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		drop solution was mixed with an equal volume of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	11 (mg/ml)
2	1	reservoir	ammonium sulfate	1.5-1.7 (M)
3	1	reservoir	PEG400	1 (%)
4	1	reservoir	sodium formate	20 (mM)
5	1	reservoir	HEPES/NaOH	100 (mM)