1FDN
REFINED CRYSTAL STRUCTURE OF THE 2[4FE-4S] FERREDOXIN FROM CLOSTRIDIUM ACIDURICI AT 1.84 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 34.440, 34.440, 74.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 1.840 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.580 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 * |
| Total number of observations | 15645 * |
| Number of reflections | 4203 * |
| Completeness [%] | 92.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 281 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 55-60 (%sat) | |
| 2 | 1 | reservoir | TrisMaleate | 100 (mM) | |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | drop | ammonium sulfate | 55-60 (%sat) | |
| 5 | 1 | drop | TrisMaleate | 100 (mM) |
