1FDL

CRYSTALLOGRAPHIC REFINEMENT OF THE THREE-DIMENSIONAL STRUCTURE OF THE FAB D1.3-LYSOZYME COMPLEX AT 2.5-ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	56.000, 143.500, 49.300
Unit cell angles	90.00, 120.40, 90.00

Refinement procedure

Resolution	? - 2.500
R-factor	0.184 *
Rwork	0.184
RMSD bond length	0.013
RMSD bond angle	3.300
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	5.500 *
High resolution limit [Å]	2.500 *
Rmerge	2.500 *
Total number of observations	41026 *
Number of reflections	20475 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *		referred to J.Mol.Biol. 170.1055-1058 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	FabD1.3-HEL	10-15 (mg/ml)
2	1	reservoir	PEG6000	20-22.5 (%(w/v))
3	1	reservoir	potassium phosphate	0.1 (M)