1FCQ
CRYSTAL STRUCTURE (MONOCLINIC) OF BEE VENOM HYALURONIDASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-28 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.886, 90.229, 49.060 |
| Unit cell angles | 90.00, 92.36, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.600 |
| R-factor | 0.192 |
| Rwork | 0.185 |
| R-free | 0.22700 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.022 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.038 | 0.251 |
| Number of reflections | 43930 | |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 96.3 | 88 |
| Redundancy | 2.3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 * | 20 * | PEG 6000, NaCl, acetic acid, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium acetate | 5 (mM) | |
| 2 | 1 | drop | protein | 8-10 (mg/ml) | |
| 3 | 1 | reservoir | PEG6000 | 10 (%) | |
| 4 | 1 | reservoir | 1.0 (M) | ||
| 5 | 1 | reservoir | acetate | 20 (mM) |
