1FCE

PROCESSIVE ENDOCELLULASE CELF OF CLOSTRIDIUM CELLULOLYTICUM

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	288
Detector technology	IMAGE PLATE
Collection date	1996-07
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	61.450, 84.540, 121.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.163 *
Rwork	0.165
R-free	0.21000
Structure solution method	MIRAS
RMSD bond length	0.009
RMSD bond angle	1.465 *
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.073 *	0.248 *
Number of reflections	43593
<I/σ(I)>	8	3
Completeness [%]	99.9	100
Redundancy	4.2	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	293 *	Reverbel-Leroy, C., (1997) Acta Crystallogr., D.54, 114. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	IG4	2 (mM)
3	1	drop	PEG4000
4	1	reservoir		0.5 (M)	or 0.3M