1FCA
STRUCTURE OF THE FERREDOXIN FROM CLOSTRIDIUM ACIDURICI: MODEL AT 1.8 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 34.441, 34.441, 74.778 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.800 |
| R-factor | 0.143 |
| Rwork | 0.143 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 5.000 | |
| High resolution limit [Å] | 1.800 | |
| Number of reflections | 4500 | 3921 * |
| Completeness [%] | 80.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | 20 * | Murthy, H. M. K., (1988) J. Biol. Chem., 263, 18430. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 100 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 46-52 (%sat) | |
| 4 | 1 | reservoir | ammonium sulfate | 65-74 (%sat) |
