1FC5
CRYSTAL STRUCTURE OF MOLYBDOPTERIN BIOSYNTHESIS MOEA PROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-28 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.640, 101.850, 103.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.226 * |
| Rwork | 0.253 |
| R-free | 0.27700 * |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.440 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.230 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.067 * | 0.209 * |
| Total number of observations | 232716 * | |
| Number of reflections | 40862 * | |
| <I/σ(I)> | 19.6 | |
| Completeness [%] | 98.2 | 88.3 |
| Redundancy | 5.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 293 | used macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 15 (%(w/v)) | |
| 2 | 1 | reservoir | 100 (mM) | ||
| 3 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 4 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | ethylene glycol | 1 (%(v/v)) | |
| 6 | 1 | drop | protein | 10 (mg/ml) | |
| 7 | 1 | drop | Tris | 10 (mM) | |
| 8 | 1 | drop | dithiothreitol | 5 (mM) |
