1FBX
CRYSTAL STRUCTURE OF ZINC-CONTAINING E.COLI GTP CYCLOHYDROLASE I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 227.690, 314.190, 132.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.980 - 2.800 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.25100 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.120 | |
| Total number of observations | 572885 * | |
| Number of reflections | 107321 * | |
| Completeness [%] | 90.2 | 44.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | PEG 6000, KCL, MOPS, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 10 (mM) | |
| 3 | 1 | drop | 0.02 (%) | ||
| 4 | 1 | reservoir | PEG6000 | 6 (%) | |
| 5 | 1 | reservoir | 150 (mM) | ||
| 6 | 1 | reservoir | 4-morpholineprpanesulfonic | 100 (mM) |
