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1FBT

THE BISPHOSPHATASE DOMAIN OF THE BIFUNCTIONAL RAT LIVER 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE

Experimental procedure
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X12C
Synchrotron siteNSLS
BeamlineX12C
Temperature [K]95
Detector technologyIMAGE PLATE
Collection date1994-10-04
DetectorMARRESEARCH
Spacegroup nameP 1 21 1
Unit cell lengths48.400, 56.600, 93.700
Unit cell angles90.00, 94.40, 90.00
Refinement procedure
Resolution6.000

*

- 2.500

*

R-factor0.208

*

Rwork0.218
R-free0.27300

*

RMSD bond length0.007

*

RMSD bond angle1.400

*

Data reduction softwareDENZO
Phasing softwareX-PLOR
Refinement softwareX-PLOR
Data quality characteristics
 Overall
Low resolution limit [Å]10.000

*

High resolution limit [Å]2.500

*

Rmerge0.068
Number of reflections15137

*

Completeness [%]93.7
Redundancy6.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, hanging drop

*

7

*

Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein7.5 (mg/ml)
101dropwater
21dropPEG400015 (%)
31dropsodium phosphate5 (mM)
41dropdithiothreitol0.5 (mM)
51dropglycerol2.5 (%)
61reservoirPEG400030 (%)
71reservoirsodium phosphate10 (mM)
81reservoirdithiothreitol1 (mM)
91reservoirglycerol5 (%)

229380

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