1FBI
CRYSTAL STRUCTURE OF A CROSS-REACTION COMPLEX BETWEEN FAB F9.13.7 AND GUINEA-FOWL LYSOZYME
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.700, 195.500, 50.200 |
| Unit cell angles | 90.00, 108.50, 90.00 |
Refinement procedure
| Resolution | 7.000 - 3.000 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.650 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 3.000 * |
| Rmerge | 0.140 * |
| Total number of observations | 111931 * |
| Number of reflections | 30232 * |
| Completeness [%] | 95.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.5 * | 17 * | Lescar, J., (1993) Proteins.Struct.,Funct., Genet., 15, 209. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 10 (%(w/v)) | |
| 2 | 1 | reservoir | sodium azide | 0.04 (%) | |
| 3 | 1 | reservoir | potassium phosphate | 0.1 (M) | |
| 4 | 1 | reservoir | magnesium chloride | 10 (mM) | |
| 5 | 1 | reservoir | 2-propanol | 3 (%(v/v)) |
