1FB6

CRYSTAL STRUCTURE OF THIOREDOXIN M FROM SPINACH CHLOROPLAST (OXIDIZED FORM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-20
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1997-03-31
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	74.710, 74.710, 74.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.600 - 2.100
R-factor	0.204 *
Rwork	0.204
R-free	0.23000
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.600	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.086	0.396
Number of reflections	14256
<I/σ(I)>	10.2
Completeness [%]	99.3	92
Redundancy	8.5	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3 *	20 *	sodium acetate, PEG monomethylether 2000, ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	50 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop		0.02 (%(w/v))
4	1	reservoir	ammonium sulfate	0.2 (M)
5	1	reservoir	sodium acetate	0.1 (M)
6	1	reservoir	PEG2000 mme	30 (%(w/v))