1FB0

CRYSTAL STRUCTURE OF THIOREDOXIN M FROM SPINACH CHLOROPLAST (REDUCED FORM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-20
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1997-04-12
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	74.600, 74.600, 74.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.400 - 2.260
R-factor	0.198 *
Rwork	0.198
R-free	0.23500
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.400	2.310
High resolution limit [Å]	2.260	2.260
Rmerge	0.070	0.269
Number of reflections	10890
<I/σ(I)>	10
Completeness [%]	94.3	91.7
Redundancy	1.9	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3 *	20 *	sodium acetate, PEG monomethylether 2000, ammonium sulphate , pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	50 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop		0.02 (%(w/v))
4	1	reservoir	ammonium sulfate	0.2 (M)
5	1	reservoir	sodium acetate	0.1 (M)
6	1	reservoir	PEG2000 mme	30 (%(w/v))