1FAI

THREE-DIMENSIONAL STRUCTURE OF TWO CRYSTAL FORMS OF FAB R19.9, FROM A MONOCLONAL ANTI-ARSONATE ANTIBODY

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	42.000, 80.600, 75.200
Unit cell angles	90.00, 90.20, 90.00

Refinement procedure

Resolution	6.000 * - 2.700
Rwork	0.189
RMSD bond length	0.014
RMSD bond angle	3.500
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.500 *	2.500 *
Rmerge	0.080 *
Total number of observations	41617 *
Number of reflections	16492 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.2 *		took 9 from original paper *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	potassium phosphate	0.02 (M)
3	1	reservoir	PEG6000	25 (%(w/v))
4	1	reservoir	pottassium phosphate	0.1 (M)
5	1	reservoir		3 (mM)
6	1	reservoir		0.3 (M)