1FA9
HUMAN LIVER GLYCOGEN PHOSPHORYLASE A COMPLEXED WITH AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1994-07-19 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 123.910, 123.910, 127.680 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.221 |
Rwork | 0.235 |
R-free | 0.29500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gpa |
RMSD bond length | 0.011 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.057 | 0.495 |
Number of reflections | 53400 * | |
<I/σ(I)> | 22.9 | |
Completeness [%] | 98.5 | 91.8 |
Redundancy | 9 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 * | 298 | Peg 8000, Tris/HCl, AMP, glucose, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 25K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-20 (mg/ml) | |
2 | 1 | drop | NaBES | 20 (mM) | |
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | drop | dithiothreitol | 0.5 (mM) | |
5 | 1 | drop | glucose | 50 (mM) | |
6 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
7 | 1 | reservoir | PEG8000 | 12 (%) |