1FA8

CRYSTAL STRUCTURE OF THE APO FORM GLYOXALASE I OF ESCHERICHIA COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-05-20
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	46.275, 57.197, 46.990
Unit cell angles	90.00, 95.24, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.188 *
Rwork	0.188
R-free	0.25500
RMSD bond length	0.006
RMSD bond angle	1.483
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.800
High resolution limit [Å]	1.700	1.700
Rmerge	0.051	0.241 *
Total number of observations	81372 *
Number of reflections	29051
<I/σ(I)>	11.5
Completeness [%]	97.8	93.1
Redundancy	2.8	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5	275	PEG 1000, PEG 4000, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 275K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12-37 (mg/ml)
2	1	reservoir	PEG1000	5-10 (%)
3	1	reservoir	PEG8000	5-10 (%)