1FA2
CRYSTAL STRUCTURE OF BETA-AMYLASE FROM SWEET POTATO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-08-15 |
| Detector | FUJI |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 129.630, 129.630, 68.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.660 - 2.300 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.28100 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.500 * |
| Data reduction software | WEIS |
| Data scaling software | WEIS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 35046 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.06 * | 298 | 20% Polyethyleneglycol 1500, 50 mM CaCl2, 150 mM sodium 4-touene sulfonate, pH 6.5, vapor diffusion, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 10 | 1 | reservoir | 0.02 (%(w/v)) | ||
| 11 | 1 | reservoir | maltose | 20 (mM) | |
| 12 | 1 | reservoir | PIPES | 100 (mM) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | 2-mercaptoethanol | 1 (mM) | |
| 5 | 1 | drop | EDTA | 0.1 (mM) | |
| 6 | 1 | reservoir | PEG1500 | 20 (%(w/v)) | |
| 7 | 1 | reservoir | 50 (mM) | ||
| 8 | 1 | reservoir | sodium 4-toluene sulfonate | 0.02 (%(w/v)) | |
| 9 | 1 | reservoir | DL-dithithreitol | 1 (mM) |
