1F9K
WINGED BEAN ACIDIC LECTIN COMPLEXED WITH METHYL-ALPHA-D-GALACTOSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-20 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 182.110, 182.110, 44.990 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WINGED BEAN BASIC LECTIN |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.110 | 0.442 * |
| Total number of observations | 47085 * | |
| Number of reflections | 11152 | |
| Completeness [%] | 98.9 | 98.2 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.2 | 298 | Manoj, N., (1999) Acta Crystallogr., D55, 564. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 25 (mg/ml) | |
| 2 | 1 | 1 | sugar | in phosphate buffer(20-fold molar excess) | |
| 3 | 1 | 1 | PEG4000 | 30 (%(w/v)) |
