1F9J
STRUCTURE OF A NEW CRYSTAL FORM OF TETRAUBIQUITIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-03-01 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 97.020, 97.020, 88.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.700 |
| R-factor | 0.224 * |
| Rwork | 0.225 |
| R-free | 0.29200 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.920 | 2.870 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.136 | 0.383 |
| Total number of observations | 27007 * | |
| Number of reflections | 5846 | |
| <I/σ(I)> | 6 | |
| Completeness [%] | 96.3 | 96.6 * |
| Redundancy | 4.6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | lithium sulfate, ammonium sulfate, sodium citrate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 10 | 1 | drop | peptide | 1 (mM) | |
| 2 | 1 | drop | ammonium acetate | 0.5 (mM) | |
| 3 | 1 | drop | EDTA | 0.001 (mM) | |
| 4 | 1 | drop | 5 (mg/ml) | ||
| 5 | 1 | drop | dithiothreitol | 1 (mM) | |
| 6 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 7 | 1 | reservoir | ammonium sulfate | 0.4 (M) | |
| 8 | 1 | reservoir | 1.05 (M) | ||
| 9 | 1 | drop | Ub4 | 0.44 (mM) |
